FUNCTIONAL CHARACTERIZATION OF MOUNTAIN MICROORGANISMS (BIOLES) AND THEIR EFFECTS ON Eryngium foetidum YIELD
Keywords:
Biol, culantro, organic fertilizer, plant growth promoters, sequencing.Abstract
Mountain microorganisms (MM) have been applied artisanally as organic fertilizers in agricultural soils, obtaining promising results. The objectives of this research were: to characterize the bacteria present in a biol obtained from mountain soils, to quantify the performance as a fertilizer of the coyote culantro crop (Eryngium foetidum L.) and to determine its optimal dose. The field work was carried out in the community of Bajo Bonito, located in Capira, Panamá Oeste. The experimental design was a randomized complete block, with six treatments (biol dose) and four blocks. The chlorophyll level and yield (kg/treatment) were evaluated. The statistical analysis concluded that treatment 5 (100 ml biol/17.90 L water) was the most efficient for the fertilization of culantro. The soil studied, not being treated with agrochemicals, maintains its structure and function, so it turned out to be suitable for agricultural production. Regarding laboratory work, 34 strains were isolated, which were subjected to different biochemical tests, proving to be efficient in promoting plant growth, with siderophore producers standing out among all the groups. Additionally, the following strains were identified: Lysinibacillus fusiformis, Bacillus kochii, Methylobacterium sp. and Bacillus velezensis, through 16S ribosomal RNA gene sequencing. The application of bioles is presented as a sustainable alternative for the environment, as well as for small and medium-sized farmers seeking to improve their crop yields and provide a higher quality product to consumers.
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References
Álvarez-Solís, J. D., Gómez-Velasco, D. A., León-Martínez, N. S., & Gutiérrez-Miceli, F. A. (2010). Manejo integrado de fertilizantes y abonos orgánicos en el cultivo de maíz. Agrociencia, 44(5), 575-586. https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000500007
Arora, N. K., & Verma, M. (2017). Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria. Biotech, 7, 381. https://link.springer.com/article/10.1007/s13205-017-1008-y
Barrera, G. (2016). Análisis y caracterización de sideróforos producidos por rizobacterias para el control de la antracnosis causada por Colletotrichum gloeosporioides en frutos de aguacate. [Tesis de Maestría en Ciencias. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional]. https://repositorio.cinvestav.mx/bitstream/handle/cinvestav/1578/SSIT0014029.pdf?sequence=1
Beltrán-Pineda, M. E. (2014). La solubilización de fosfatos como estrategia microbiana para promover el crecimiento vegetal. Ciencia y Tecnología Agropecuaria, 15(1), 101-113. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-87062014000100009&lng=en&tlng=es
Bruner, G., Jimenez, L., Mendoza, Y., Chial, B., & de Chial, M. (2013). Bacterial Diversity and Physicochemical Stratification of the Water Column during the Rainy Season in Las Cumbres Lake, Panama. The Internet Journal of Microbiolog, 12(1). http://ispub.com/IJMB/12/1/14553
Cárdenas, M., & Hondoy, D. (2017). Efecto del biol como fertilizante orgánico en tres cultivares de Pennisetum purpureum. [Tesis de Ingeniero Zootecnista. Universidad Nacional Agraria]. https://www.sidalc.net/search/Record/dig-una-ni-3610/Description; https://repositorio.una.edu.ni/3610/1/tnf04c266e.pdf
Chandra, S., Askari, K., & Kumari, M. (2018). Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth. Journal of Genetic Engineering and Biotechnology, 16(2), 581-586. https://doi.org/10.1016/j.jgeb.2018.09.001
Cisneros-Rojas, C. A., & Sánchez de Prager, M. (2015). Solubilización de fosfatos por hongos asociados a un Andisol de tres agroecosistemas cafeteros de la región andina colombiana. Ingenium, 9(25), 37-46. https://repositorio.usc.edu.co/server/api/core/bitstreams/61e79143-e202-4a19-926a-a632e68f403a/content
Cycon, M., Żmijowska, A., Wójcik, M., & Piotrowska-Seget, Z. (2013). Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils. Journal of Environmental Management, 117, 7-16. https://doi.org/10.1016/j.jenvman.2012.12.031
Escobar, C., Horna, Y., Carreño, C., & Mendoza, G. (2011). Caracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en Lambayeque. Scientia Agropecuaria, 2(1), 39-49. https://doi.org/10.17268/sci.agropecu.2011.01.05
Espinosa-Tasón, J., & Barba, A. (2014). Reseña histórica de la problemática de contaminación por agroquímicos y mecanismos de regulación en Panamá. Visión Antataura, 2(1), 37-48. https://core.ac.uk/reader/478586890
Google Earth. (2022). Capira, Panamá. Google Earth. https://earth.google.com/web/@8.72500002,-80.05600001,455.51180484a,1000.40187406d,30y,0h,0t,0r/data=MikKJwolCiExcDRCZEViNWxzLTJVLXlEY2p0RkdCVXU2d0o4VF9aN1cgAToDCgEw?authuser=0
Hernández, B., Cornejo, H., & Mejía., F. (2016). Manual de laboratorio del Curso de Microbiología Ambiental de suelos. Panamá: Universidad de Panamá.
Holdridge, L. (1987). Ecología: basada en zonas de vida. IICA, 216. https://books.google.es/books?hl=es&lr=&id=oXutTpUKUb4C&oi=fnd&pg=PA54&dq=Holdridge,+L.+(1987).+Ecolog%C3%ADa:+basada+en+zonas+de+vida.+IICA,+216.&ots=7MCM3S_mim&sig=ijcSbAiIvPHVkTsd9TYTCyF-tLA#v=onepage&q&f=false
Ibarra, J. (2016). Caracterización de bacterias solubilizadoras de fósforo nativas de los suelos de Sinaloa con potencial para incrementar el crecimiento y mejorar la nutrición fosforada del maíz (Zea mays L.). [Tesis de maestría en recursos naturales y medio ambiente. Instituto Politécnico Nacional].
Kloepper, J., Leong, W., Teintze, M., & Schroth, M. N. (1980). Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature, 885-886. https://www.nature.com/articles/286885a0
Lebrazi, S., Fadil, M., Chraibi, M., & Fikri-Benbrahim, K. (2020). Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology. Journal of Genetic Engineering and Biotechnology, 18(1), 1-10. https://jgeb.springeropen.com/articles/10.1186/s43141-020-00035-9
Lindsay, W. L., Vlek, P. L., & Chien, S. H. (1989). Phosphate minerals. Minerals in soil, 1089-1130. https://doi.org/10.2136/sssabookser1.2ed.c22
Loper, J. E., & Schroth, M. N. (1986). Importance of siderophores in microbial interactions in the Rhizosphere. Iron, Siderophores, and Plant Diseases 117, 85-98. https://link.springer.com/chapter/10.1007/978-1-4615-9480-2_11
Mantilla, M. (2007). Evaluación de la acción de un bioinoculante sobre un cultivo de crisantemo (Chrysanthemun morifolium var. yoko ono) en período de enraizamiento. [Tesis de Microbiología Agrícola. Pontificia Universidad Javeriana, Bogotá, Colombia]. http://hdl.handle.net/10554/8381
Milagres, A., Machuca, A., & Napoleão, D. (1999). Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. Journal of Microbiological Methods, 37(1), 1-6. https://doi.org/10.1016/S0167-7012(99)00028-7
Mohite, B. (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of soil science and plant nutrition, 13(3), 638-649. https://www.scielo.cl/scielo.php?pid=S0718-95162013005000051&script=sci_arttext&tlng=en
Molina-Romero, D., Bustillos-Cristales, M. R., Rodríguez-Andrade, O., Morales-García, Y. E., Santiago-Saenz, Y., Castañeda-Lucio, M., & Muñoz-Rojas, J. (2015). Mecanismos de fitoestimulación por rizobacterias, aislamientos en América y potencial biotecnológico. Biológicas, 17(2), 24-34. https://www.biologicas.umich.mx/index.php?journal=biologicas&page=article&op=view&path%5B%5D=207&path%5B%5D=pdf
Morales, J., Brunner, B., Flores, L., & Martínez, S. (2013). Hoja Informativa: culantro orgánico. Lajas, Puerto Rico : Proyecto de agricultura orgánica. Departamento de cultivos y ciencias agroambientales.Estación Experimental Agrícola de Lajas. Obtenido de Proyecto de agricultura orgánica. Departamento de cultivos y ciencias agroambientales.
Ozdal, M., Gur, O., Sezen, A., & Faruk, O. (2017). Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis. Revista 3 Biotech, 7, 23. https://link.springer.com/article/10.1007/s13205-017-0605-0
Parfitt, R. L., & Kimble, J. M. (1989). Conditions for formation of allophane in soils. Soil Science Society of America Journal, 53(3), 971-977. https://doi.org/10.2136/sssaj1989.03615995005300030057x
Patten, C. L., & Glick, B. R. (2002). Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and environmental microbiology, 68(8), 3795-3801. https://journals.asm.org/doi/full/10.1128/aem.68.8.3795-3801.2002
https://doi.org/10.1128/AEM.68.8.3795-3801.2002
Payne, S. M. (1993). Iron acquisition in microbial pathogenesis. Trends in microbiology, 1(2), 66-69. https://www.cell.com/trends/microbiology/abstract/0966-842X(93)90036-Q
Restrepo-Franco, G. M., Marulanda-Moreno, S., de la Fe-Pérez, Y., Díaz-de la Osa, A., Lucia-Baldani, V., & Hernández-Rodríguez, A. (2015). Bacterias solubilizadoras de fosfato y sus potencialidades de uso en la promoción del crecimiento de cultivos de importancia económica. Revista CENIC Ciencias Biológicas, 46(1), 63-76. https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1010376/1/BacteriassolubilizadorasdefosfatoysuspotencialidadesdeusoBaldani.pdf
Rodríguez-Chávez, J. (11 de Marzo de 2020). Microorganismos benéficos en la agricultura. Recuperado el 27 de Agosto de 2024, de Centro Universitario CEICKOR.: https://www.centrouniversitarioceickor.edu.mx/home/2020/01/10/microorganismos-beneficos-en-la-agricultura/
Salazar, A. M., & Ordoñez, C. A. (2013). Aislamiento de identificación de actinomicetos fijadores denitrógeno en el suelo del Jardín Botánico de la Universidad Tecnológica de Pereira. [Trabajo de Grado, Química Industrial, Universidad Tecnológica de Pereira] 115p. https://repositorio.utp.edu.co/entities/publication/97aa4803-79d1-4a48-9957-4279d0444230
Salazar, J., & Aldana, M. (2011). Herbicida glifosato: usos, toxicidad y regulación. Biotecnia, 13(2), 23-28. https://www.redalyc.org/pdf/6729/672971155003.pdf
Sanyal, S. K., & De Datta, S. K. (1991). Chemistry of phosphorus transformations in soil. . Advances in soil science, 1-120. https://link.springer.com/chapter/10.1007/978-1-4612-3144-8_1
Schwyn, B., & Neilands, J. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160(1), 47-56. https://doi.org/10.1016/0003-2697(87)90612-9
Spaepen, S., Vanderleyden, J., & Okon, Y. (2009). Chapter 7 Plant growth-promoting actions of rhizobacteria. Advances in Botanical Research, 51, 283-320. https://doi.org/10.1016/S0065-2296(09)51007-5
Suchini-Ramírez, J. (2012). Innovaciones agroecológicas para una producción agropecuaria sostenible en la región del Trifinio. Centro Agronómico Tropical de Investigación y Enseñanza (CATIE). https://repositorio.catie.ac.cr/handle/11554/7790
Tomer, S., Suyal, D. C., & Goel, R. (2016). Biofertilizers: A timely approach for sustainable agriculture. Plant-Microbe Interaction: An Approach to Sustainable Agriculture, 375-395. doi:10.1007/978-981-10-2854-0_17. https://www.researchgate.net/publication/314263435_Biofertilizers_A_Timely_Approach_for_Sustainable_Agriculture
Tripti, K., Kumar, A., & Anshumali, V. (2012). Hosphate Solubilizing Activity of Some Bacterial Strains Isolated from Chemical Pesticide Exposed Agriculture Soil. International Journal of Engineering Research and Development. https://www.researchgate.net/publication/282323814_Phosphate_Solubilizing_Activity_of_Some_Bacterial_Strains_Isolated_from_Chemical_Pesticide_Exposed_Agriculture_Soil
Villachica, H. (1996). Frutales y hortalizas promisorios de la Amazônia. Lima: Tratado de Cooperacion Amazônica, 385. https://agris.fao.org/search/en/providers/122452/records/6472488908fd68d546009bfb
Zepeda-Jazo, I. (2018). Manejo sustentable de plagas agrícolas en México. Agricultura, sociedad y desarrollo, 15(1), 23-28. https://www.scielo.org.mx/scielo.php?pid=S1870-54722018000100099&script=sci_arttext
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