FIRST REPORT OF THE ENTOMOPATHOGENIC NEMATODE Heterorhabditis indica Poinar, 1992 (Rhabditida: Heterorhabditidae) IN PANAMA
Keywords:
Bioprospecting, biological control, morphometric identification, molecular characterization, native isolate.Abstract
A bioprospecting survey conducted by the Nematology Laboratory of the Instituto de Innovación Agropecuaria de Panamá (IDIAP-NEMALAB) in Colón Province, Panama, resulted in the discovery of a native strain of an entomopathogenic nematode, morphologically identified as belonging to the genus Heterorhabditis. Entomopathogenic nematodes are recognized as effective biological control agents against larval stages of numerous insect pests, and their efficacy has been demonstrated worldwide, including in preliminary trials conducted by NEMALAB at the Eastern Agricultural Innovation Center (CIAOr) of IDIAP in Tanara, Chepo District, Eastern Panama. Species identification of the native isolate, experimentally designated NEMALAB 1H, was further investigated through detailed morphometric analyses of hermaphroditic females, amphimictic females, males, and third-stage infective juveniles (IJ3). The morphometric characteristics obtained were consistent with those previously reported for Heterorhabditis indica Poinar, 1992. Molecular analyses subsequently confirmed the morphological identification. This study constitutes the first confirmed record of H. indica in Panama and provides the basis for future research on its potential use in biological control programs.
Downloads
References
Abd-Elgawad, M. M. M. (2024). Reproduction of Entomopathogenic Nematodes for Use in Pest Control. Methods in molecular biology (Clifton, N.J.), 2756, 351-382. https://doi.org/10.1007/978-1-0716-3638-1_14
Bedding, R. A. & Akhurst, R. J. (1975). A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21, 109-110. https://www.cabidigitallibrary.org/doi/full/10.5555/19760541046
Candanedo-Lay, E., Aranda-Caballero, G., Cabezón-Puchicama, A., & Reina-Peña, L. (2020). Bioprospección y conservación de cepas nativas del nematodo entomopatógeno Heterorhabditis en Panamá. Ciencia Agropecuaria, (30), 139-149. http://www.revistacienciaagropecuaria.ac.pa/index.php/ciencia-agropecuaria/article/view/133
Hazir, S., Kaya, H. K., Stock, S. P., & Keskin, N. (2003). Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae) for Biological Control of Soil Pests. Turkish Journal of Biology, 27, 181-202. https://journals.tubitak.gov.tr/biology/vol27/iss4/1/
Grewal, P. S., De Nardo, E. A. B., & Aguilera, M. M. (2001). Entomopathogenic nematodes: potential for exploration and use in South America. Nematropical Entomology, 30 (2), 191-205. https://doi.org/10.1590/S1519-566X2001000200001 https://www.scielo.br/j/ne/a/Ws7fJJkwyBK8YYCHmf8bKxg/?lang=en
Grifaldo-Alcántara, P., Alatorre-Rosas, R., Villanueva-Jiménez, J., Hernández-Rosas, F., Stock, S. P., & Ramírez-Valverde, G. (2019). Evaluación de dos cepas de nematodos entomopatógenos (Steinernematidae, Heterorhabditidae) para el control del salivazo (Hemiptera: Cercopidae) en caña de azúcar. Nematropica, 49(1), 83-90. https://www.researchgate.net/publication/338828687_Evaluacion_de_dos_cepas_de_nematodos_Steinernematidae_Heterorhabditidae_para_el_control_del_salivazo_Hemiptera_Cercopidae_en_cana_de_azucar
Grifaldo-Alcántara, P., Alatorre-Rosas, R., Silva-Rojas, H., Stock, S. P., Hernández-Rosas, F., Vargas-Madriz, H., Azuara-Domínguez, A., & Durán-Trujillo, Y. (2020). Caracterización molecular y morfométrica de Heterorhabditis indica (CEPA CP13JA) aislado en el cultivo de caña de azúcar. Tropical and Subtropical Agroecosystems, 23(2), 1 - 10. http://dx.doi.org/10.56369/tsaes.3278
Joyce, S., Reid, A., Driver, F., & Curran, J. (1994). Application of polymerase chain reaction (PCR) methods to the identification of entomopathogenic nematodes. Tropical and Subtropical Agroecosystems 23 (2020): #64
Grifaldo-Alcantara et al., 2020. In: Burnell, A.M., Ehlers, R.U., and Masson, J.P. (eds.). Biotechnology: Genetics of entomopathogenic nematodes bacterium complexes. Proceedings of symposium and workshop, St Patrick’s College, Maynooth, County Kildare, Ireland. Luxembourg, European Commission, DGXII: 178-187. https://www.cabidigitallibrary.org/doi/full/10.5555/19952307151
Lawrence, A. L., & Georgis, R. (2012). Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial productction. Journal of Nematology, 44(2), 218-225. https://pubmed.ncbi.nlm.nih.gov/23482993/
Meza-García, J., Elías-Santos, M., Corte-Mondaca, E., Guerrero-Olazarán, M., Viader-Salvadó, J., Luna-Olvera, H., Maldonado-Blanco, M., Quintero-Zapata, I., & Pereyra-Alférez, B. (2014). Evaluation of Heterorhabditis indica (Rhabditida: Heterorhabditidae) Nematode Strain from Sinaloa, Mexico, Against Bemisia tabaci Immatures Under Laboratory Conditions. Southwestern Entomologist, 39(4), 727-738. https://doi.org/10.3958/059.039.0404
Nguyen, K. B., & Hunt, D. (2007). Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. 816 pp. Brill. https://books.google.com.pa/books/about/Entomopathogenic_Nematodes_Systematics_P.html?id=Xz6xCQAAQBAJ&redir_esc=y
Poinar, Jr., George, O., Karunakar, G. K., & Hastings, D. (1992). Heterorhabditis indica n. sp. (Rhabditida: Nematoda) from India: Separation of Heterorhabditis spp. by infective juveniles. Fundam. Applicate Nematology, 15, 467-472. https://www.researchgate.net/publication/32977705_Heterorhabditis_indicus_n_sp_Rhabditida_Nematoda_from_India_Separation_of_Heterorhabditis_spp_by_infective_juveniles
Sánchez-Saavedra, M. G., Cortez-Madrigal, H., & Cristóbal-Acevedo, D. (2012). Infectividad de Heterorhabditis indica (Rhabditida: Heterorhabditidae) en adultos y larvas de gallina ciega (Coleoptera: Melolonthidae). Revista Chapingo Serie Horticultura, 18(3), 383-394. https://doi.org/10.5154/r.rchsh.2012.08.040
Stack, C., Easwaramoorthy, S., Metha, U., Downes, M., Griffin, C., & Burnell, A. (2000). Molecular characterization of Heterorhabditis indica isolates from India, Kenya, Indonesia and Cuba. Nematology, 2(5), 477-487.
Downloads
Published
Issue
Section
License
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional.
